|Test Name||Vibrio Stool Culture|
|Preferred Container||Cary-Blair Transport Media|
|Other Specimen/Container||Rectal Swab in Cary-Blair Transport, Stool in Clean|
Container, Rectal Swab in Bacterial Transport Container (e.g. Culturette)
|Optimum Volume||Stool: 5 gm|
Rectal Swab: 2 Swabs
|Collection Instructions||Antacids, barium, bismuth, antidiarrheal medication or oily laxatives prior to collection should not be used|
Rectal Swab: Collect anorectal specimens from the crypts just inside the anal ring. Indicate specimen source on the requisition.
|Handling Instructions||Stool, Buffered (Cary-Blair): The sample should include any blood or mucus present. Use the plastic spoon provided in the cap of the Cary- Blair container to transfer 5 grams of sample into the buffered solution. The liquid should come up to the "fill" line on the vial. Tightly cap the vial and shake firmly to insure that the specimen is adequately mixed with the solution|
Stool, Unbuffered: The sample should include any blood or mucus present
Place sample in a clean container. Refrigerate immediately
Rectal Swab: Place swabs in Cary-Blair transport or in Bacterial Transport Container (e.g. Culturette).
|Transport Requirements||Stool, Buffered (Cary-Blair): Oahu: Ambient,|
Stool, Unbuffered: Oahu: Refrigerated, Airline: Refrigerated
Rectal Swab: Oahu: Ambient, Airline: Ambient
|Specimen Stability||Stool, Buffered (Cary-Blair): Ambient: 4 Days, Refrigerated: 4 Days|
Stool, Unbuffered: Ambient: 2 Hours, Refrigerated: 24 Hours
Rectal Swab in Cary-Blair: Ambient: 4 Days, Refrigerated: 4 Days
Rectal Swab in Bacterial Transport: Ambient: 2 Days, Refrigerated: 2 Days
|Rejection Criteria||Frozen, Received in Formalin and/or PVA, Specimen older than stability limits, Stool contaminated with urine, Dry cotton swab received, Stool in diaper or tissue paper, 24 hour collection or pooled specimens|
|Analytic Time||Up to 3 Days|
Additional time is required for identification if culture is positive.
|Methodology||Visual examination for bacterial growth on selective media.|
The reference ranges listed below are valid on this date of April 20, 2021.
|Component||Age||Male Norm||Male Critical Low||Male Critical High||Female Norm||Female Critical High||Female Critical Low||Units||Add'l info|
|Culture Result:||ALL||See report.|