Test directory: Vancomycin (Trough)

Ordering Code340T
Test NameVancomycin (Trough)
AliasTrough Vancocin
Preferred SpecimenPlasma
Preferred ContainerEDTA, K2 (Lavender)
Other Specimen/ContainerPlasma in Lithium Heparin (Green), Serum in Plain (Red)
Optimum Volume0.5 mL
Collection InstructionsCollect specimen just prior to the next dose.
Handling InstructionsQMC Draws: Transport EDTA tube to the QMC Lab within 2 hours of collection
Other Draws: Plasma in EDTA or Lithium Heparin/Serum in
Plain (Red): Send aliquot.
Transport RequirementsOahu: Refrigerated
Airline: Frozen
Specimen StabilityAmbient: 48 Hours (aliquot)
Refrigerated: 14 Days (aliquot)
Frozen: 12 Months (aliquot)
Frozen samples should be thawed only once.
Rejection CriteriaGrossly hemolyzed, Specimen older than stability limits,
Uncentrifuged unaliquoted Plasma in EDTA or Lithium Heparin/
Serum in Plain (Red) specimens >2 hours old
Avail. StatYES
Analytic Time1 Day
MethodologyKinetic interaction of microparticles (KIMS)

Reference range(s)

ComponentAgeMale NormMale Critical LowMale Critical HighFemale NormFemale Critical HighFemale Critical LowUnitsAdd'l info
Vancomycin (Trough)ALL10-2010-20ug/mL
Therapeutic Range: ... Therapeutic Range:
Trough: = 10 - 20 ug/mL
Minimum serum trough concentration should be above 10 ug/mL to avoid development of resistance. A trough concentration of 15 - 20 ug/mL is recommended for complicated infections caused by S. Aureus in which the MIC to Vancomycin is 1
(Am J Health-Syst Pharm 2017; 74 (14): 1067-1075)