Test directory: Phenobarbital

Ordering Code 441
Test Name Phenobarbital
Alias Luminal
Barbita
Solfoton
PBARB
Preferred Specimen Plasma
Preferred Container Lithium Heparin PST (Green)
Other Specimen/Container Plasma in EDTA, K2 (Lavender), Serum in SST (Gold), Serum in
Plain (Red)
Optimum Volume 1 mL
Collection Instructions It is recommended to measure the steady-state concentration representing the trough level just before the next scheduled dose.
Handling Instructions All specimen types must be centrifuged within 2 hours of collection. If no gel separation present in tube and testing is delayed, plasma/serum is aliquoted from cells within 2 hours of collection
Send aliquot if transport time >24 hours.
Transport Requirements Oahu: Refrigerated
Airline: Refrigerated (Aliquot)
Specimen Stability Refrigerated: 24 Hours (Centrifuged, unaliquoted gel separator tubes), 1 Month (Aliquot)
Frozen: 3 Months (Aliquot)
Frozen samples should be thawed only once
Test cannot be added to >24 hours unaliquoted specimens collected in a gel separator tube.
Rejection Criteria Grossly hemolyzed, Insufficient quantity, Specimen older than stability limits, Uncentrifuged gel separator tube specimens >2 hours old, Unaliquoted gel separator tube specimens >24 hours old
Avail. Stat YES
Analytic Time 1 Day
Methodology Homogenous Enzyme Immunoassay (EMIT)

Reference range(s)

Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
Phenobarbital ALL 10.0-40.0 >= 50.0 10.0-40.0 >= 50.0 ug/mL