Test directory: HLA-B27

Ordering Code 458
Test Name HLA-B27
Alias B27
Preferred Specimen Whole Blood
Preferred Container Sodium Heparin (Green)
Other Specimen/Container Whole Blood in ACD-B (Yellow),
Whole Blood in EDTA, K2 (Lavender),
Whole Blood in Sodium Heparin (Green)
Optimum Volume 6 mL
Collection Instructions Draw full tube
Mix tube(s) well.
Handling Instructions Do NOT refrigerate or freeze
Do NOT centrifuge specimen
For optimal results, deliver to testing laboratory within 24 hours of collection.
Transport Requirements Oahu: Ambient
Airline: Ambient
Specimen Stability Na Heparin or ACD: 72 Hours (For optimal results: 24 hours ambient for all specimen types)
Rejection Criteria Clotted, Refrigerated or Frozen
Avail. Stat NO
Analytic Time Up to 3 Days
Methodology Flow Cytometry
Reference Lab  Hawaii Pathologist Laboratory

Reference range(s)

The reference ranges listed below are valid on this date of June 1, 2025.
Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
HLA-B27 ALL

HLA-B27...
HLA-B27 antigen is present in 8% of Caucasians, 1-3% of
Asians and 3% of African Americans. In patients with ankylosing spondylitis, HLA-B27 is found in 89%, 85% and 58% of Caucasians, Asians and African Americans, respectively
HLA-B27 is found in 80% of Caucasians with Reiter's disease and 25% of juvenile rheumatoid arthritis patients

Flow cytometric evaluation of HLA-B27 is a screening tool and should be verified with a second method (microcytotoxicity or molecular method) to confirm positive or equivocal results

Test performed by the Queen's Medical Center, Pathology Department

See report.
Note1

Note1:


HLA-B27 antigen is present in 8% of Caucasians, 1-3% of
Asians and 3% of African Americans. In patients with ankylosing spondylitis, HLA-B27 is found in 89%, 85% and 58% of Caucasians, Asians and African Americans, respectively
HLA-B27 is found in 80% of Caucasians with Reiter's disease and 25% of juvenile rheumatoid arthritis patients

Flow cytometric evaluation of HLA-B27 is a screening tool and should be verified with a second method (microcytotoxicity or molecular method) to confirm positive or equivocal results

Test performed by the Queen's Medical Center, Pathology Department

See report..