Test directory: HIV-1 RNA, Quant

Ordering Code4609
Test NameHIV-1 RNA, Quant
AliasHuman Immunodeficiency Virus
HIV-1 Viral Load
Preferred SpecimenPlasma
Preferred ContainerEDTA, K2 (Lavender)
Optimum Volume2 mL
Collection InstructionsCannot share with any other test!
Handling InstructionsSeparate plasma from whole blood within 24 hours of collection. Use only polypropylene tubes for aliquot samples. DO NOT thaw once plasma is frozen.
Transport RequirementsOahu: Refrigerated
Airline: Frozen
Specimen StabilityRefrigerated: 6 Days
Frozen: 12 Weeks
Rejection CriteriaSpecimen older than stability limits, Unspun and unseparated specimen > 24 hours, Plasma other than EDTA, Frozen specimen thawed, Non-polypropylene aliquot tube
Avail. StatNO
Analytic TimeUp to 7 Days
MethodologyReal-Time PCR

Reference range(s)

ComponentAgeMale NormMale Critical LowMale Critical HighFemale NormFemale Critical HighFemale Critical LowUnitsAdd'l info

The linear...

The linear range for the cobas (R) HIV-1 for 500 uL sample processing volume is from 20 cp/mL to 1.00E+07 cp/mL (33.3 IU/mL to 1.67E+07 IU/mL)

The limit of detection (LoD) for the cobas (R) HIV-1 using
EDTA plasma is 13.2 cp/mL (22.0 IU/mL) for a 500 uL sample processing volume

Reliable results depend on proper sample collection, storage and handling procedures. Quantitation of HIV-1 RNA is dependent on the number of virus particles present in the samples and may be affected by sample collection methods
HIV-1 RNAALL<20<20cpy/mL
HIV-1 RNAALL<1.3<1.3log cpy/mL