| Ordering Code | 4609 |
|---|---|
| Test Name | HIV-1 RNA, Quant |
| Alias | Human Immunodeficiency Virus HIV-1 Viral Load |
| Preferred Specimen | Plasma |
| Preferred Container | EDTA, K2 (Lavender) |
| Optimum Volume | 2 mL |
| Collection Instructions | Cannot share with any other test! |
| Handling Instructions | Separate plasma from whole blood within 24 hours of collection. Use only polypropylene tubes for aliquot samples. DO NOT thaw once plasma is frozen. |
| Transport Requirements | Oahu: Refrigerated Airline: Frozen |
| Specimen Stability | Refrigerated: 6 Days Frozen: 12 Weeks |
| Rejection Criteria | Specimen older than stability limits, Unspun and unseparated specimen > 24 hours, Plasma other than EDTA, Frozen specimen thawed, Non-polypropylene aliquot tube |
| Avail. Stat | NO |
| Analytic Time | Up to 7 Days |
| Methodology | Real-Time PCR |
Reference range(s)
The reference ranges listed below are valid on this date of October 3, 2023.
| Component | Age | Male Norm | Male Critical Low | Male Critical High | Female Norm | Female Critical High | Female Critical Low | Units | Add'l info |
|---|---|---|---|---|---|---|---|---|---|
| HIV-1 RNA, Quant | ALL | NOT DETECTED | NOT DETECTED | The linear... Note1 | |||||
| HIV-1 RNA | ALL | <20 | <20 | cpy/mL | |||||
| HIV-1 RNA | ALL | <1.3 | <1.3 | log cpy/mL |
Note1:
The linear range for the cobas (R) HIV-1 for 500 uL sample processing volume is from 20 cp/mL to 1.00E+07 cp/mL (33.3 IU/mL to 1.67E+07 IU/mL)
The limit of detection (LoD) for the cobas (R) HIV-1 using
EDTA plasma is 13.2 cp/mL (22.0 IU/mL) for a 500 uL sample processing volume
Reliable results depend on proper sample collection, storage and handling procedures. Quantitation of HIV-1 RNA is dependent on the number of virus particles present in the samples and may be affected by sample collection methods
.