Test directory: HIV-1 RNA, Quant

Ordering Code 4609
Test Name HIV-1 RNA, Quant
Alias Human Immunodeficiency Virus
HIV-1 Viral Load
Preferred Specimen Plasma
Preferred Container EDTA, K2 (Lavender)
Optimum Volume 2 mL
Collection Instructions Cannot share with any other test!
Handling Instructions Separate plasma from whole blood within 24 hours of collection. Use only polypropylene tubes for aliquot samples. DO NOT thaw once plasma is frozen.
Transport Requirements Oahu: Refrigerated
Airline: Frozen
Specimen Stability Refrigerated: 6 Days
Frozen: 12 Weeks
Rejection Criteria Specimen older than stability limits, Unspun and unseparated specimen > 24 hours, Plasma other than EDTA, Frozen specimen thawed, Non-polypropylene aliquot tube
Avail. Stat NO
Analytic Time Up to 7 Days
Methodology Real-Time PCR

Reference range(s)

Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
HIV-1 RNA, Quant ALL NOT DETECTED NOT DETECTED

The linear...

The linear range for the cobas (R) HIV-1 for 500 uL sample processing volume is from 20 cp/mL to 1.00E+07 cp/mL (33.3 IU/mL to 1.67E+07 IU/mL)

The limit of detection (LoD) for the cobas (R) HIV-1 using
EDTA plasma is 13.2 cp/mL (22.0 IU/mL) for a 500 uL sample processing volume

Reliable results depend on proper sample collection, storage and handling procedures. Quantitation of HIV-1 RNA is dependent on the number of virus particles present in the samples and may be affected by sample collection methods
HIV-1 RNA ALL <20 <20 cpy/mL
HIV-1 RNA ALL <1.3 <1.3 log cpy/mL