| Ordering Code | 479 |
|---|---|
| Test Name | Neisseria gonorrhoeae PCR |
| Alias | GC, PCR N.gonorrhoeae, PCR GC Nucleic Acid Amplification |
| Preferred Specimen | Female: Urine/Vaginal/Endocervical Male: Urine Female & Male: Anorectal/Oropharyngeal |
| Preferred Container | Vaginal/ Endocervical swab: Cobas PCR swab kit Urine: Cobas PCR urine kit or Raw Urine Oropharyngeal Swab: Cobas PCR swab kit Anorectal Swab: Cobas PCR swab kit |
| Other Specimen/Container | Urethral swab/ Penile swab/ Vaginal swab/ Endocervical swab in UTM |
| Optimum Volume | Raw Urine: 6 mL, Cobas PCR Swab kit: Entire Specimen, Cobas PCR Swab Kit: 1 swab Universal Transport Media (UTM): 3 mL |
| Collection Instructions | Use the swabs provided in the Cobas PCR Swab kit ONLY Indicate source on requisition Endocervical: Remove excess mucous & blood from the cervix using the LARGER swab provided with the kits and discard swab Insert the smaller swab into the endocervix canal and rotate the swab 5 times Place small swab in the Cobas PCR media Break the shaft at the scoreline, and cap the tube securely Vaginal: Insert the LARGER swab about 2 inches into the vaginal opening and rotate the swab for about 30 seconds Place LARGER swab in the Cobas PCR media Break the shaft at the scoreline, and cap the tube securely Discard the small swab Male Urethral: Patient should NOT have urinated for at least 1 hour prior to specimen collection Insert the LARGER swab 2-4 cm into the urethra and rotate for 3-5 seconds Place the LARGE swab in the Cobas PCR media Break the shaft at the scoreline, and cap the tube securely. Discard the small swab Urine: Patient should not have urinated for at least 1 hour prior to specimen collection Collect first portion of a voided urine in a sterile polypropylene container Requires a separate urine container for this test (cannot be shared with non-PCR). |
| Handling Instructions | Raw Urine: If Cobas Urine kit is available, use the provided pipet to transfer urine into the Cobas PCR media tube Fill raw urine to the level between the two black lines on the tube label. |
| Transport Requirements | Oahu: Raw Urine: Refrigerated Cobas PCR Urine Kit: Ambient Cobas PCR Swab Kit: Ambient Universal Transport Media (UTM): Refrigerated Airline: Raw Urine: Refrigerated Cobas PCR Urine Kit: Ambient Cobas PCR Swab Kit: Ambient Universal Transport Media (UTM): Refrigerated |
| Specimen Stability | Raw Urine: Ambient 1 Day, Refrigerated: 7 Days, Frozen: 1 Month Cobas PCR Urine Kit: Ambient: 12 Months, Refrigerated: 12 Months Cobas PCR Swab Kit: Ambient: 12 Months, Refrigerated: 12 Months Universal Transport Media (UTM): Ambient: 12 Hours, Refrigerated: 7 Days, Frozen: 1 Month |
| Rejection Criteria | Specimen exceeds stability limits, Cotton swab, Wooden-shafted or aluminum-shafted swabs No swab or evidence of inoculation in collection media Specimen from children <14 years of age. |
| Avail. Stat | NO |
| Analytic Time | 3 Days |
| Methodology | Real-Time PCR |
Reference range(s)
The reference ranges listed below are valid on this date of December 3, 2024.
| Component | Age | Male Norm | Male Critical Low | Male Critical High | Female Norm | Female Critical High | Female Critical Low | Units | Add'l info |
|---|---|---|---|---|---|---|---|---|---|
| Source | ALL | See report. | |||||||
| GC, PCR | ALL | NEGATIVE | NEGATIVE |