Test directory: Hepatitis C RNA, Quant.

Ordering Code5442
Test NameHepatitis C RNA, Quant.
AliasHCV RNA Quantitative
HCV Viral Load
Preferred SpecimenSerum
Preferred ContainerSST (Gold)
Other Specimen/ContainerPlasma in EDTA, K2 (Lavender), Serum in Plain (Red)
Optimum Volume2 mL
Collection InstructionsCannot share with any other test!
Handling InstructionsSeparate serum/plasma from whole blood within 24 hours of collection. Use only polypropylene tubes for aliquot samples. DO NOT thaw once serum/plasma is frozen.
Transport RequirementsOahu: Refrigerated
Airline: Frozen
Specimen StabilityRefrigerated: 6 Days
Frozen: 12 Weeks
Rejection CriteriaSpecimen older than stability limits, Unspun & unseparated specimen >6 hours, Plasma other than EDTA, Frozen specimen thawed, Non-polypropylene aliquot tube
Avail. StatNO
Analytic TimeUp to 7 Days
MethodologyReal time PCR

Reference range(s)

ComponentAgeMale NormMale Critical LowMale Critical HighFemale NormFemale Critical HighFemale Critical LowUnitsAdd'l info

The linear...

The linear range for the cobas (R) HCV for EDTA plasma and serum is from 15 IU/mL to 1.00E+08 IU/mL and the accuracy of the test is within +/-0.24 log10. The lower limit of quantification (LLoQ) is 15 IU/mL

The limit of detection (LoD) of cobas (R) HCV was determined for WHO International Standard (genotype 1a) and for genotype 1b through 6. The overall LoD was 12.0 IU/mL for EDTA plasma and 13.7 IU/mL for serum

Reliable results depend on proper sample collection, storage and handling procedures. Quantitation of HCV RNA is dependent on the number of virus particles present in the samples and may be affected by sample collection methods, patient factors (i.e. age, presence of symptoms), and/or stage of infection
HCV RNAALL<1.2<1.2Log IU/mL