Test directory: B. pertussis/parapertussis DNA

Ordering Code5607
Test NameB. pertussis/parapertussis DNA
AliasBordetella pertussis/parapertussis
DNA, Qualitative, Real-Time PCR
Preferred SpecimenNasopharyngeal swab
Preferred ContainerViral Transport Medium (M4, VCM, UTM, Eswab)
Other Specimen/ContainerNasopharyngeal Aspirate in sterile vial
Polyester, rayon, or nylon swab tips are acceptable
Flexible shafts must be used for proper collection.
Optimum Volume1 mL
Collection InstructionsNasopharyngeal swab: Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for a few seconds for optimal collection. Collection of both nostrils is recommended but not required

Nasopharyngeal aspirates: Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril. Flush a plastic catheter or tubing with 2-3 mL of saline. Insert the tubing into the nostril parallel to the palate
Aspirate nasopharyngeal secretions. Repeat this procedure for the other nostril. Combine aspirates into a sterile vial.
Transport RequirementsOahu: Refrigerated
Airline: Frozen
Specimen StabilityNasopharyngeal swab:
Ambient: 7 Days
Refrigerated: 7 Days
Frozen: 30 Days
Nasopharyngeal Aspirate:
Ambient: 2 Days
Refrigerated: 8 Days
Frozen: 30 Days
Rejection CriteriaCalcium alginate swab, Cotton swab, Collection tubes with
Amies gel or charcoal containing media, Rigid shaft swabs
Avail. StatNO
Analytic Time5 Days
MethodologyReal-Time Polymerase Chain Reaction (RT-PCR)
Reference Lab Quest Diagnostics Infectious Disease

Reference range(s)

ComponentAgeMale NormMale Critical LowMale Critical HighFemale NormFemale Critical HighFemale Critical LowUnitsAdd'l info
SourceALLSee report.
B. Pertussis DNAALLSee report.
B. Parapertussis DNAALLSee report.