Test directory: Vitamin D, 25-Hydroxy (D2 & D3)

Ordering Code 5679
Test Name Vitamin D, 25-Hydroxy (D2 & D3)
Alias 25-Hydroxy-Vitamin D3
25-Hydroxy Vitamin D2
Vitamin D2, 25-Hydroxy
Vitamin D3, 25-Hydroxy
Preferred Specimen Serum
Preferred Container SST (Gold)
Other Specimen/Container Serum in Plain (Red)
Optimum Volume 1 mL
Collection Instructions Allow to clot at room temp., centrifuge and remove from gel within 48 hours. Fasting specimen preferred but not required.
Handling Instructions Separate serum promptly
Freeze and thaw acceptable.
Transport Requirements Oahu: Ambient
Airline: Ambient
Specimen Stability Ambient: 3 Weeks
Refrigerated: 3 Weeks
Frozen: 3 Weeks
Rejection Criteria Grossly hemolyzed, grossly lipemic, grossly icteric, Serum not separated from SST gel or not clotted within 48 hours,
Samples out of stability range
Avail. Stat NO
Analytic Time Up to 5 Days
Methodology Liquid Chromatography Tandem mass Spectrometry
Reference Lab  Quest Diagnostic Lab-NI

Reference range(s)

The reference ranges listed below are valid on this date of June 1, 2025.
Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
Vitamin D, 25-OH, Total ALL See report.
Vitamin D, 25-OH, D3 ALL See report.
Vitamin D, 25-OH, D2 ALL See report.