Test directory: Blood Culture

Ordering Code 619
Test Name Blood Culture
Preferred Specimen Blood
Preferred Container BacT/ALERT blood culture bottles (Aerobic & Anaerobic for adults, Pediatric for pediatric)
Other Specimen/Container Whole Blood in SPS tubes or Bactec standard aerobic, anaerobic or peds glass culture bottles
Optimum Volume Adult: BacT/Alert bottles: 10 mL, Bactec aerobic: 10 mL, Bactec anaerobic: 7 mL
Pediatric: BacT/Alert bottles: 4 mL, Bactec bottle: 3 mL
Collection Instructions Clean with 70% isopropyl or ethyl alcohol. Follow with 2% iodine, allow to remain on the site for two minutes
If patient is allergic to iodine, double swab with alcohol and allow to air dry
It is strongly recommended both aerobic and anaerobic bottles are submitted for optimal organism recovery.
Transport Requirements Oahu: Ambient
Airline: Ambient
Specimen Stability Ambient: BacT/Alert bottles: 1 Day Bactect bottles: 2 Days
Rejection Criteria Frozen, refrigerated, exposed to excessive heat, Defective or damaged bottle(s)
While not optimal, receipt of blood culture bottles beyond stability will be tested and reported with a disclaimer.
Avail. Stat NO
Analytic Time 5 Days
Final report upon positive isolation or at 5 days (when no growth).
Methodology Detection of increases of CO2 production by the growth of microorganisms.

Reference range(s)

Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
Source: ALL See report.
Status: ALL See report.
Culture: ALL See report.