|Test Name||Blood Culture|
|Preferred Container||BacT/ALERT blood culture bottles (Aerobic & Anaerobic for adults, Pediatric for pediatric)|
|Other Specimen/Container||Whole Blood in SPS tubes or Bactec standard aerobic, anaerobic or peds glass culture bottles|
|Optimum Volume||Adult: BacT/Alert bottles: 10 mL, Bactec aerobic: 10 mL, Bactec anaerobic: 7 mL|
Pediatric: BacT/Alert bottles: 4 mL, Bactec bottle: 3 mL
|Collection Instructions||Clean with 70% isopropyl or ethyl alcohol. Follow with 2% iodine, allow to remain on the site for two minutes|
If patient is allergic to iodine, double swab with alcohol and allow to air dry
It is strongly recommended both aerobic and anaerobic bottles are submitted for optimal organism recovery.
|Transport Requirements||Oahu: Ambient|
|Specimen Stability||Ambient: BacT/Alert bottles: 1 Day Bactect bottles: 2 Days|
|Rejection Criteria||Frozen, refrigerated, exposed to excessive heat, Defective or damaged bottle(s)|
While not optimal, receipt of blood culture bottles beyond stability will be tested and reported with a disclaimer.
|Analytic Time||5 Days|
Final report upon positive isolation or at 5 days (when no growth).
|Methodology||Detection of increases of CO2 production by the growth of microorganisms.|
The reference ranges listed below are valid on this date of March 25, 2023.
|Component||Age||Male Norm||Male Critical Low||Male Critical High||Female Norm||Female Critical High||Female Critical Low||Units||Add'l info|