|Test Name||Anaerobic Culture|
|Alias||Anaerobic Culture, Abscess|
Anaerobic Culture, Wound
Abscess Culture, Anaerobic
Anaerobic Culture, Body Fluid
Anaerobic Culture, Tissue Culture
|Preferred Specimen||Body Fluid, Aspirates and Tissue|
|Preferred Container||Body Fluid and Aspirates: Capped syringe, with needle removed and air expelled.|
|Other Specimen/Container||**Swabs are not optimal, but will accept Eswab or anaerobic transport media**|
|Optimum Volume||Aspirates: 3 mL|
Sterile Body Fluids (CSF, Pleural Fld., etc.): 5 mL
Tissue: 1 gm
Any Body Site Swab: 2 Swabs
|Collection Instructions||Specimen site must be from areas without normal anaerobic flora. Collect aseptically. Indicate specimen source on the requisition.|
|Handling Instructions||Specimen must be placed immediately in Eswab or anaerobic transport. If sending specimen in syringe, expel the air, remove the needle and cap syringe securely.|
|Transport Requirements||Oahu: Ambient|
|Specimen Stability||Ambient: 2 Days|
Anaerobic condition must be maintained.
|Rejection Criteria||Specimen older than stability limits, Inappropriate site for anaerobic culture, Non-anaerobic conditions, |
Refrigerated, Frozen, syringe with needle attached
Note: specimens with rejection concerns that cannot be recollected (e.g. surgical tissue) will be processed upon receipt and the ordering physician consulted.
|Analytic Time||Up to 4 Days|
Preliminary culture results reported at 48 hours. If culture grows, additional time is needed for identification.
|Methodology||Visual examination for bacterial growth on selective and non-selective media.|