Test directory: Acid Fast Smear and Culture Sterile

Ordering Code651S
Test NameAcid Fast Smear and Culture Sterile
AliasAFB Smear and Culture
Mycobacteria smear and culture
TB smear and culture
MAI smear and culture
NTM smear and culture
Preferred SpecimenTissue, Sterile body fluids (CSF, Pleural fluid, etc)
Preferred ContainerSterile container
Optimum VolumeSterile Body Fluids (CSF, Pleural fluid, etc): 5 mL
Tissue: 1 g
Collection InstructionsIf large volume of fluid is collected, send a 10 mL aliquot of a well-mixed specimen. If a syringe is to be submitted, remove needle and use a luer-tip to cap the syringe before submitting to the laboratory.
Handling InstructionsRequests on isolates submitted by other clients are to be ordered as order code 4534. An indentification will be performed and charged if an organism ID is not provided
Ensure agar slant is appropriate for the type of organism submitted. Culture must be viable, pure, and safely contained (for air transport, agar plate is not acceptable & potentially biohazardous). Air transported specimens must be packaged according to infectious shipment regulations. A processing fee will be assessed if mixed isolates are submitted.
Transport RequirementsOahu: Refrigerated
Airline: Refrigerated
Specimen StabilityAmbient: 2 Hours
Refrigerated: 5 Days
CSF is also stable at 33-37 degrees C for 5 days.
Rejection CriteriaFrozen, Pooled specimen, Preservatives used, Specimen older than stability limits, Syringe with needle attached
Note: Specimens with rejection concerns that cannot be recollected (e.g. surgical tissue) will be processed upon receipt and the ordering physician consulted.
Avail. StatNO
Analytic TimeUp to 8 Weeks
Smear result available within 24 hours. Positives are reported as soon as detected. Additional time is required for ID/susceptibility if culture is positive.
MethodologyGrowth detection by CO2 production of acid-fast organism & microscopic examination of Fluorescent/carbol Fuchsin stained smear

Reference range(s)

ComponentAgeMale NormMale Critical LowMale Critical HighFemale NormFemale Critical HighFemale Critical LowUnitsAdd'l info
Site:ALLSee report.
Acid Fast Smear:ALLSee report.
AFB Culture Status:ALLSee report.
AFB Culture:ALLSee report.