|Test Name||Acid Fast Smear and Culture Sterile|
|Alias||AFB Smear and Culture|
Mycobacteria smear and culture
TB smear and culture
MAI smear and culture
NTM smear and culture
|Preferred Specimen||Tissue, Sterile body fluids (CSF, Pleural fluid, etc)|
|Preferred Container||Sterile container|
|Optimum Volume||Sterile Body Fluids (CSF, Pleural fluid, etc): 5 mL|
Tissue: 1 g
|Collection Instructions||If large volume of fluid is collected, send a 10 mL aliquot of a well-mixed specimen. If a syringe is to be submitted, remove needle and use a luer-tip to cap the syringe before submitting to the laboratory.|
|Handling Instructions||Requests on isolates submitted by other clients are to be ordered as order code 4534. An indentification will be performed and charged if an organism ID is not provided|
Ensure agar slant is appropriate for the type of organism submitted. Culture must be viable, pure, and safely contained (for air transport, agar plate is not acceptable & potentially biohazardous). Air transported specimens must be packaged according to infectious shipment regulations. A processing fee will be assessed if mixed isolates are submitted.
|Transport Requirements||Oahu: Refrigerated|
|Specimen Stability||Ambient: 2 Hours|
Refrigerated: 5 Days
CSF is also stable at 33-37 degrees C for 5 days.
|Rejection Criteria||Frozen, Pooled specimen, Preservatives used, Specimen older than stability limits, Syringe with needle attached|
Note: Specimens with rejection concerns that cannot be recollected (e.g. surgical tissue) will be processed upon receipt and the ordering physician consulted.
|Analytic Time||Up to 8 Weeks|
Smear result available within 24 hours. Positives are reported as soon as detected. Additional time is required for ID/susceptibility if culture is positive.
|Methodology||Growth detection by CO2 production of acid-fast organism & microscopic examination of Fluorescent/carbol Fuchsin stained smear|
The reference ranges listed below are valid on this date of April 14, 2021.
|Component||Age||Male Norm||Male Critical Low||Male Critical High||Female Norm||Female Critical High||Female Critical Low||Units||Add'l info|
|Acid Fast Smear:||ALL||See report.|
|AFB Culture Status:||ALL||See report.|
|AFB Culture:||ALL||See report.|