| Ordering Code | 7600 |
|---|---|
| Test Name | KRAS Mutation Analysis |
| Alias | Kirsten Rat Sarcoma Viral Oncogene Homolog Gene Analysis |
| Preferred Specimen | Formalin-Fixed, Paraffin Embedded Tissue (FFPE) |
| Preferred Container | 1.5 mL tubes |
| Other Specimen/Container | Fresh Tissue, Frozen Tissue |
| Optimum Volume | 6-10 Sections of tissue at 6 microns |
| Handling Instructions | Each slide needs to be H&E stained and coverslip removed One slide needs to be evaluated for tumor and necrosis content. The tumor needs to be circled for microdissection. |
| Transport Requirements | Oahu or Airline: Paraffin Embedded Tissue: Ambient Fresh Tissue: Refrigerated or Frozen (if previously Frozen) Formalin-Fixed, Paraffin Embedded Tissue: Ambient |
| Specimen Stability | Ambient: 7 Days for Paraffin Embedded Tissue Not Stable for Fresh or Frozen Tissue Refrigerated: 3 Days for Fresh Tissue Frozen: 10 Days for Fresh Tissue |
| Rejection Criteria | Tumor content below 30% and decalcified samples |
| Avail. Stat | NO |
| Analytic Time | 10 Days |
| Methodology | Real-Time PCR |
Reference range(s)
The reference ranges listed below are valid on this date of April 20, 2021.
| Component | Age | Male Norm | Male Critical Low | Male Critical High | Female Norm | Female Critical High | Female Critical Low | Units | Add'l info |
|---|---|---|---|---|---|---|---|---|---|
| KRAS Mutation Analysis | ALL | See report. |