| Ordering Code | 7603 |
|---|---|
| Test Name | BRAF Mutation Analysis |
| Alias | B-Raf proto-oncogene |
| Preferred Specimen | Formalin-Fixed, Paraffin Embedded Tissue (FFPE) |
| Preferred Container | 1.5 mL tubes |
| Optimum Volume | 6-10 Sections of Tissue @ 6 microns |
| Handling Instructions | Each slide needs to be H&E stained and coverslip removed One slide needs to be evaluated for tumor and necrosis content. The tumor needs to be circled for microdissection. |
| Transport Requirements | Oahu or Airline: Paraffin Embedded Tissue: Ambient Fresh Tissue: Refrigerated or Frozen (if previously Frozen) Formalin-Fixed, Paraffin Embedded Tissue: Ambient (Do NOT send Fresh Tissue) |
| Specimen Stability | Ambient: Paraffin Embedded Tissue: 7 Days Fresh or Frozen Tissue: Not Stable Refrigerated: Fresh Tissue: 3 Days Frozen: Fresh Tissue: 10 Days |
| Rejection Criteria | Tumor content below 30% and decalcified samples |
| Avail. Stat | NO |
| Analytic Time | 10 Days |
| Methodology | Real-Time PCR |
Reference range(s)
The reference ranges listed below are valid on this date of December 9, 2022.
| Component | Age | Male Norm | Male Critical Low | Male Critical High | Female Norm | Female Critical High | Female Critical Low | Units | Add'l info |
|---|---|---|---|---|---|---|---|---|---|
| BRAF Mutation Analysis | ALL | See report. |