Test directory: BRAF Mutation Analysis

Ordering Code7603
Test NameBRAF Mutation Analysis
AliasB-Raf proto-oncogene
Preferred SpecimenFormalin-Fixed, Paraffin Embedded Tissue (FFPE)
Preferred Container1.5 mL tubes
Optimum Volume6-10 Sections of Tissue @ 6 microns
Handling InstructionsEach slide needs to be H&E stained and coverslip removed
One slide needs to be evaluated for tumor and necrosis content. The tumor needs to be circled for microdissection.
Transport RequirementsOahu or Airline:
Paraffin Embedded Tissue: Ambient
Fresh Tissue: Refrigerated or Frozen (if previously Frozen)
Formalin-Fixed, Paraffin Embedded Tissue: Ambient (Do NOT
send Fresh Tissue)
Specimen StabilityAmbient:
Paraffin Embedded Tissue: 7 Days
Fresh or Frozen Tissue: Not Stable
Fresh Tissue: 3 Days
Fresh Tissue: 10 Days
Rejection CriteriaTumor content below 30% and decalcified samples
Avail. StatNO
Analytic Time10 Days
MethodologyReal-Time PCR

Reference range(s)

ComponentAgeMale NormMale Critical LowMale Critical HighFemale NormFemale Critical HighFemale Critical LowUnitsAdd'l info
BRAF Mutation AnalysisALLSee report.