Test directory: Estradiol

Ordering Code 771
Test Name Estradiol
Alias E2
** Note: STAT for approved fertility physicians only **
Preferred Specimen Serum
Preferred Container SST (Gold)
Other Specimen/Container Serum in Plain (Red), Plasma in Lithium Heparin (Green-PST),
Plasma in Lithium Heparin (Dark Green-PP No Gel)
Optimum Volume 1 mL
Collection Instructions ** Note: STAT for approved fertility physicians only **
Handling Instructions All specimen types must be centrifuged within 2 hours of collection. If no gel separation present in tube and testing is delayed, plasma/serum is aliquoted from cells within 2 hours of collection
Keep tubes stoppered at all times.
Transport Requirements Oahu: Refrigerated
Airline: Refrigerated
Specimen Stability Ambient: 8 Hours
Refrigerated: 7 Days
Frozen: 6 Months
Frozen samples should not be thawed more than 3 times
DO NOT use frost-free freezers.
Rejection Criteria Hemolysis, Lipemia, Insufficient quantity, Specimens older than stability limits, Wrong specimen type
Avail. Stat NO
Analytic Time 1 Day
Methodology Chemiluminescent Immunoassay-Competitive

Reference range(s)

The reference ranges listed below are valid on this date of November 4, 2025.
Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
Estradiol >=19Y <31.6 pg/mL

  ...
                       Male >=19 Y:         =        <31.6 pg/mL
                       Female >=19 Y:
                        Early Follicular    = 22.4 - 115.0 pg/mL
                        Mid Follicular      = 25.0 - 115.0 pg/mL
                        Ovulatory Peak      = 32.1 - 517.0 pg/mL
                        Mid Luteal          = 36.5 - 246.0 pg/mL
                        Postmenopause       =        <25.2 pg/mL
                       Pediatric:
                        Pediatric Male 0-1Y       =  <38.3 pg/mL
                        Pre-puberty Male 1-11Y    =  <15.0 pg/mL
                        Puberty Male 12-18Y       =  <34.9 pg/mL
                        Pediatric Female 0-1Y     =  <38.3 pg/mL
                        Pre-puberty Female 1-11Y  =  <16.1 pg/mL
                        Puberty Female 12-18Y     =  <196.1 pg/mL
Note1
Estradiol 0-18Y pg/mL

  ...
                       Male >=19 Y:         =        <31.6 pg/mL
                       Female >=19 Y:
                        Early Follicular    = 22.4 - 115.0 pg/mL
                        Mid Follicular      = 25.0 - 115.0 pg/mL
                        Ovulatory Peak      = 32.1 - 517.0 pg/mL
                        Mid Luteal          = 36.5 - 246.0 pg/mL
                        Postmenopause       =        <25.2 pg/mL
                       Pediatric:
                        Pediatric Male 0-1Y       =  <38.3 pg/mL
                        Pre-puberty Male 1-11Y    =  <15.0 pg/mL
                        Puberty Male 12-18Y       =  <34.9 pg/mL
                        Pediatric Female 0-1Y     =  <38.3 pg/mL
                        Pre-puberty Female 1-11Y  =  <16.1 pg/mL
                        Puberty Female 12-18Y     =  <196.1 pg/mL

See report.
Note1

Note1:


                       Male >=19 Y:         =        <31.6 pg/mL
                       Female >=19 Y:
                        Early Follicular    = 22.4 - 115.0 pg/mL
                        Mid Follicular      = 25.0 - 115.0 pg/mL
                        Ovulatory Peak      = 32.1 - 517.0 pg/mL
                        Mid Luteal          = 36.5 - 246.0 pg/mL
                        Postmenopause       =        <25.2 pg/mL
                       Pediatric:
                        Pediatric Male 0-1Y       =  <38.3 pg/mL
                        Pre-puberty Male 1-11Y    =  <15.0 pg/mL
                        Puberty Male 12-18Y       =  <34.9 pg/mL
                        Pediatric Female 0-1Y     =  <38.3 pg/mL
                        Pre-puberty Female 1-11Y  =  <16.1 pg/mL
                        Puberty Female 12-18Y     =  <196.1 pg/mL
.