Test directory: Chromosome, Blood, Routine

Ordering Code8332
Test NameChromosome, Blood, Routine
Routine G-Banding
Preferred SpecimenWhole blood
Preferred ContainerSodium Heparin (Green)
Optimum Volume5 mL (adults), 1-2 mL (neonates)
Collection InstructionsVenipuncture: Thoroughly cleanse the area with a
Betadine scrub or similar preparation followed by a rinse with 70% alcohol (ethanol may be used instead of isopropyl and is less toxic). Collect 1-10 mL of blood into a heparinized tube. Invert the tube several times to prevent coagulation
If a Vacutainer is used, let off any remaining vacuum by inserting a hypodermic needle or loosening the top briefly

Skin lancet puncture: Thoroughly cleanse the site (finger, earlobe, or newborn child's heel) by the above method. Rinse a sterile Pasteur pipet or TB syringe with approximately 0.5 mL of sterile heparin, and aspirate blood into it.
Care must be taken to use only blood within the sterilized site; dripping blood may be removed with a sterile gauze pad. The blood-heparin mixture may be placed into a sterile tube for transport

Specimens must be sent the same day as collected and arrive in the laboratory Monday through
Saturday and within 72 hours of collection.
Handling InstructionsPertinent medical findings must accompany request for chromosome analysis. Include the patient's name, age, and suspected diagnosis
Specimens may be refrigerated if there is a delay in shipment. Do NOT freeze.
Transport RequirementsOahu: Refrigerate
Airline: Refrigerate
Specimen should be sent to the laboratory IMMEDIATELY.
Rejection CriteriaHemolysis, Specimen clotted / use of improper anticoagulant,
Frozen specimen
Avail. StatNO
Analytic Time9 - 16 Days
MethodologyLymphocyte culture with mitogen stimulation, colchicine arrest of cells in mitosis with predominantly early metaphases, methanol/acetic acid fixation, spread preparation, trypsin-
Giemsa banding, chromosome analysis of 20 metaphases with preparation and analysis of multiple karyotypes. Other banded preparations are made as needed. Fluorescence in situ hybridization (FISH) ordered as needed (see test 510770).
Reference Lab Esoterix Genetic Laboratories, LLC

Reference range(s)

ComponentAgeMale NormMale Critical LowMale Critical HighFemale NormFemale Critical HighFemale Critical LowUnitsAdd'l info
Cells AnalyzedALLSee report.
Cells CountedALLSee report.
Cells KaryotypedALLSee report.
Cytogenetic ResultALLSee report.
Director Review:ALLSee report.
GTG Band Resolution AchievedALLSee report.
InterpretationALLSee report.
PDFALLSee report.
Specimen TypeALLSee report.