Test directory: Chromosome, Blood, Routine

Ordering Code 8332
Test Name Chromosome, Blood, Routine
Alias Karyotype
Routine G-Banding
Preferred Specimen Whole blood
Preferred Container Sodium Heparin (Green)
Optimum Volume 5 mL (adults), 1-2 mL (neonates)
Collection Instructions Venipuncture: Thoroughly cleanse the area with a
Betadine scrub or similar preparation followed by a rinse with 70% alcohol (ethanol may be used instead of isopropyl and is less toxic). Collect 1-10 mL of blood into a heparinized tube. Invert the tube several times to prevent coagulation
If a Vacutainer is used, let off any remaining vacuum by inserting a hypodermic needle or loosening the top briefly

Skin lancet puncture: Thoroughly cleanse the site (finger, earlobe, or newborn child's heel) by the above method. Rinse a sterile Pasteur pipet or TB syringe with approximately 0.5 mL of sterile heparin, and aspirate blood into it.
Care must be taken to use only blood within the sterilized site; dripping blood may be removed with a sterile gauze pad. The blood-heparin mixture may be placed into a sterile tube for transport

Specimens must be sent the same day as collected and arrive in the laboratory Monday through
Saturday and within 72 hours of collection.
Handling Instructions Pertinent medical findings must accompany request for chromosome analysis. Include the patient's name, age, and suspected diagnosis
Specimens may be refrigerated if there is a delay in shipment. Do NOT freeze.
Transport Requirements Oahu: Refrigerate
Airline: Refrigerate
Specimen should be sent to the laboratory IMMEDIATELY.
Rejection Criteria Hemolysis, Specimen clotted / use of improper anticoagulant,
Frozen specimen
Avail. Stat NO
Analytic Time 9 - 16 Days
Methodology Lymphocyte culture with mitogen stimulation, colchicine arrest of cells in mitosis with predominantly early metaphases, methanol/acetic acid fixation, spread preparation, trypsin-
Giemsa banding, chromosome analysis of 20 metaphases with preparation and analysis of multiple karyotypes. Other banded preparations are made as needed. Fluorescence in situ hybridization (FISH) ordered as needed (see test 510770).
Reference Lab  Esoterix Genetic Laboratories, LLC

Reference range(s)

The reference ranges listed below are valid on this date of May 18, 2025.
Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
Cells Analyzed ALL See report.
Cells Counted ALL See report.
Cells Karyotyped ALL See report.
Cytogenetic Result ALL See report.
Director Review: ALL See report.
GTG Band Resolution Achieved ALL See report.
Interpretation ALL See report.
PDF ALL See report.
Specimen Type ALL See report.