Test directory: Vibrio Stool Culture

Ordering Code 308
Test Name Vibrio Stool Culture
Alias Cholera Culture
Preferred Specimen Stool
Preferred Container Fecal Swab
Other Specimen/Container Stool or Rectal Swab in Cary-Blair Transport, Stool in Clean
Container
Optimum Volume Stool: 5 gm
Rectal Swab: 2 Swabs
Collection Instructions Antacids, barium, bismuth, antidiarrheal medication or oily laxatives prior to collection should not be used
Rectal Swab: Collect anorectal specimens from the crypts just inside the anal ring. Indicate specimen source on the requisition.
Handling Instructions Stool, Buffered (Cary-Blair): The sample should include any blood or mucus present. Use the plastic spoon provided in the cap of the Cary- Blair container to transfer 5 grams of sample into the buffered solution. The liquid should come up to the "fill" line on the vial. Tightly cap the vial and shake firmly to insure that the specimen is adequately mixed with the solution
Stool, Unbuffered: The sample should include any blood or mucus present
Place sample in a clean container. Refrigerate immediately
Rectal Swab: Place swabs in Cary-Blair transport or in Bacterial Transport Container (e.g. Culturette).
Transport Requirements Stool, Buffered (Cary-Blair): Oahu: Ambient,
Airline: Ambient
Stool, Unbuffered: Oahu: Refrigerated, Airline: Refrigerated
Rectal Swab: Oahu: Ambient, Airline: Ambient
Specimen Stability Stool, Buffered (Cary-Blair): Ambient: 4 Days, Refrigerated: 4 Days
Stool, Unbuffered: Ambient: 2 Hours, Refrigerated: 24 Hours
Rectal Swab in Cary-Blair: Ambient: 4 Days, Refrigerated: 4 Days
Rectal Swab in Bacterial Transport: Ambient: 2 Days, Refrigerated: 2 Days
Rejection Criteria Frozen, Received in Formalin and/or PVA, Specimen older than stability limits, Stool contaminated with urine, Dry cotton swab received, Stool in diaper or tissue paper, 24 hour collection or pooled specimens
Avail. Stat NO
Analytic Time Up to 3 Days
Additional time is required for identification if culture is positive.
Methodology Visual examination for bacterial growth on selective media.

Reference range(s)

Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
Status ALL See report.
Culture Result: ALL See report.