| Ordering Code | 6232 |
|---|---|
| Test Name | Fungus Smear by Calcofluor White |
| Alias | KOH Fungal Smear by Calcofluor White |
| Preferred Specimen | Any Body Site |
| Preferred Container | Sterile Container |
| Other Specimen/Container | Hair, Skin scrapings, Nail, Tissue, Sterile Body Fluids (CSF, Pleural, etc.) |
| Optimum Volume | Any Body Site: 5 mL of Aspirate or Eswab (or other culture swab transport) Hair: 2-3 Hair Shafts Skin Scrapings: 2-3 Skin Flakes Nail: 2-3 Pieces of Nail Tissue: 1 gm Sterile Body Fluids (CSF, Pleural Fld, etc.): 5 mL |
| Collection Instructions | Any Body Site, Tissue, Sterile Body Fluids (CSF, Pleural Fld, etc.): Indicate specimen source on the requisition Hair: Select hair which are broken off and appear diseased and pluck them with sterile forceps. If diseased hair shafts are not apparent, scrape the edges of a scalp lesion with a sterile scalpel Skin Scrapings: Cleanse skin lesions first with 70% alcohol to reduce bacteria and saprophytic fungi Scrape from outer edges of skin lesions Indicate specimen source on the requisition Nail: Scrape the friable material beneath the edge of the nails, or scrape or clip off portions of abnormal appearing nail. Do not put the specimen in cotton plugged or rubber stopper tubes. Indicate specimen source on the requisition. |
| Handling Instructions | Any Body Site, Hair, Skin Scrapings, Nail, Sterile Body Fluids (CSF, Pleural Fld, etc.): Verify with client if a culture has not been ordered with the smear Tissue: Add sterile normal saline to prevent drying. Verify with client if a culture has not been ordered with the smear. |
| Transport Requirements | Any Body Site = Oahu: Ambient, Airline: Refrigerated Hair = Oahu: Ambient, Airline: Ambient Skin Scrapings = Oahu: Ambient, Airline: Ambient Nail = Oahu: Ambient, Airline: Ambient Tissue = Oahu: Refrigerated, Airline: Refrigerated Sterile Body Fluids (CSF, Pleural Fld, etc.) = Oahu: Refrigerated, Airline: Refrigerated |
| Specimen Stability | Any Body Site = Ambient: 2 Hours, Refrigerated: 24 Hours Hair = Ambient: 3 Days, Refrigerated: 3 Days, Frozen: Not Stable Skin Scrapings = Ambient: 3 Days, Refrigerated: 3 Days, Frozen: Not Stable Nail = Ambient: 3 Days, Refrigerated: 3 Days, Frozen: Not Stable Tissue = Ambient: 2 Hours, Refrigerated: 24 Hours, Frozen: Not Stable Sterile Body Fluids (CSF, Pleural Fld, etc.) = Ambient: 2 Hours, Refrigerated: 24 Hours, Frozen: Not Stable CSF may be kept longer at 30-35 Deg C. |
| Rejection Criteria | Expired container/transport media, Leaking specimen, Dry cotton swab, No swab in media, Tissue specimen in formalin, Syringe with needle, No cellular matter on slide, Specimen older than stability limits Note: Consideration for processing will be given to rejected specimens that cannot be recollected (e.g. Surgical Tissue), based on consultation with ordering physician. |
| Avail. Stat | NO |
| Analytic Time | 1 Day |
| Methodology | Fluorescent Staining |
Reference range(s)
The reference ranges listed below are valid on this date of November 21, 2024.
| Component | Age | Male Norm | Male Critical Low | Male Critical High | Female Norm | Female Critical High | Female Critical Low | Units | Add'l info |
|---|---|---|---|---|---|---|---|---|---|
| Source: | ALL | See report. | |||||||
| Result: | ALL | See report. |