Test directory: AFB Blood/Bone Marrow

Ordering Code 646
Test Name AFB Blood/Bone Marrow
Alias AFB Blood Culture
AFB Bone Marrow Culture
MAI Blood Culture
Preferred Specimen Whole Blood or Bone Marrow
Preferred Container Bactec Myco/F Lytic Bottle
Other Specimen/Container Whole Blood or Bone Marrow in SPS (Yellow)
Optimum Volume 5 mL
For Bone Marrow, submit as much specimen as possible
Collection Instructions Note: SPS should be used only for Fungal or AFB cultures, as other bacterial organisms may be inhibited by the anticoagulant

Perform aseptic venipuncture: After palpating site, cleanse with 70% alcohol. Using tincture of iodine or provodone- iodine, swab in a circular motion concentrically from the center of the site outward. Allow iodine to dry (i.e. 2 or more minutes). DO NOT re-palpate site once it has been prepped. For iodine-sensitive patients, a double alcohol preparation may be used.
Handling Instructions The top of the culture vial or SPS anticoagulant tube must be cleansed in the same manner as the patient prior to inoculation of the blood specimen. It is NOT necessary to perform a needle change prior to inoculation of the vial or tube. Keep specimen ambient.
Transport Requirements Oahu: Ambient
Airline: Ambient
Specimen Stability Ambient: Myco/F Lytic = 16 hours from the time of collection SPS (Yellow) = 3 Days
Rejection Criteria Clotted, Frozen, Specimen older than stability limits
Note: specimens with rejection concerns that cannot be recollected (e.g. bone marrow) will be processed upon receipt and the ordering physician consulted.
Avail. Stat NO
Analytic Time Up to 6 Weeks
Additional time is required for identification &/or susceptibility testing if the culture is positive.
Methodology Culture, real time detection of increased CO2 production due to mycobacterial growth

Reference range(s)

Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
AFB Culture Status: ALL See report.
AFB Blood Result ALL See report.
AFB Bone Marrow Result ALL See report.