| Ordering Code | 6556 |
|---|---|
| Test Name | Vitamin D, 25-Hydroxy, Total |
| Alias | 25-Hydroxy Vitamin D, Total 25-OH Vitamin D, Total |
| Preferred Specimen | Serum |
| Preferred Container | SST (Gold) |
| Other Specimen/Container | Serum in Plain (Red), Plasma in Lithium Heparin (Green-PST), Plasma in Lithium Heparin (Dark Green-PP No Gel) |
| Optimum Volume | 1 mL |
| Handling Instructions | All specimen types must be centrifuged within 2 hours of collection. If no gel separation present in tube and testing is delayed, plasma/serum is aliquoted from cells within 2 hours of collection Keep tubes stoppered at all times. |
| Transport Requirements | Oahu: Refrigerated Airline: Refrigerated |
| Specimen Stability | Ambient: 8 Hours Refrigerated: 7 Days Frozen: 24 Weeks (Aliquot) Frozen samples should be thawed no more than 3 times DO NOT use frost-free freezers. |
| Rejection Criteria | Grossly Lipemic, Hemolyzed, Insufficient quantity, Specimen older than stability limits, Wrong specimen type |
| Avail. Stat | NO |
| Analytic Time | 1 Day |
| Methodology | Chemiluminescent Immunoassay - Two-step Competitive |
Reference range(s)
The reference ranges listed below are valid on this date of August 15, 2025.
| Component | Age | Male Norm | Male Critical Low | Male Critical High | Female Norm | Female Critical High | Female Critical Low | Units | Add'l info |
|---|---|---|---|---|---|---|---|---|---|
| Vitamin D, 25-Hydroxy, Total | ALL | 20.0-50.0 | 20.0-50.0 | ng/mL |