Test directory: Sequential 2

Ordering Code 8344
Test Name Sequential 2
Alias AFP, Maternal
Down Syndrome
Preferred Specimen Serum
Preferred Container SST (Gold)
Optimum Volume 5 mL
Collection Instructions Collect in serum separator tube with gel barrier. Allow blood to clot, avoiding hemolysis. Separate serum from cells by centrifugation. Transport spun tube to testing laboratory. Pour off is not advised. Maternal serum specimens must be drawn prior to amniocentesis to avoid contamination with fetal blood.
Handling Instructions For test inquiries, call CMBP genetic services at 800-345-4363. Patient must have submitted a previous specimen in the first trimester for the
Sequential 1 test. Gestational age will be based on crown rump length provided with the first trimester specimen. Patient information may be provided to the laboratory using the Maternal
Prenatal Screening test request form 0900.
Testing is provided from 15.0 to 21.9 weeks of gestation.
Transport Requirements Oahu: Ambient
Airline: Ambient
Specimen Stability Ambient: 7 Days
Refrigerated: 14 Days
Frozen: 14 Days
Rejection Criteria Gross hemolysis, Gross lipemia, Quantity not sufficient for analysis, Improper specimen type
Avail. Stat NO
Analytic Time 4 - 7 Days
Methodology Chemiluminescent immunoassay
Reference Lab  Esoterix Genetic Laboratories, LLC

Reference range(s)

The reference ranges listed below are valid on this date of November 22, 2025.
Component Age Male Norm Male Critical Low Male Critical High Female Norm Female Critical High Female Critical Low Units Add'l info
Additional US ALL See report.
AFP MoM ALL See report.
AFP Value ALL See report.
Collected On ALL See report.
Collected On ALL See report.
CRL Scan ALL See report.
CRL Scan Twin B ALL See report.
Crown Rump Length ALL See report.
Crown Rump Length Twin B ALL See report.
DIA MoM ALL See report.
DIA Value ALL See report.
Down Syndrome ALL See report.
Down Syndrome ALL See report.
Down Syndrome Interpretation ALL See report.
FIRST TRIMESTER SAMPLE ALL See report.
Gest. Age on Collection Date ALL See report.
Gestational Age ALL See report.
hCG MoM ALL See report.
hCG Value ALL See report.
Insulin Dep Diabetes ALL See report.
Maternal Age at EDD ALL See report.
Note: ALL See report.
NT MoM Twin B ALL See report.
NT Twin B ALL See report.
Nuchal Translucency (NT) ALL See report.
Nuchal Translucency MoM ALL See report.
Number of Fetuses ALL See report.
Open Spina Bifida ALL See report.
OSB Interpretation ALL See report.
PAPP-A MoM ALL See report.
PAPP-A Value ALL See report.
PDF ALL See report.
Race ALL See report.
Results ALL See report.
SECOND TRIMESTER SAMPLE ALL See report.
Sonographer ID# ALL See report.
Test Results: ALL See report.
Trisomy 18 ALL See report.
Trisomy 18 ALL See report.
Trisomy 18 Interpretation ALL See report.
uE3 MoM ALL See report.
uE3 Value ALL See report.
Weight ALL See report.
Weight ALL See report.