| Ordering Code | 8448 |
|---|---|
| Test Name | Factor V Leiden (APC Resistance V) |
| Alias | Activated Protein C Resistance APCR |
| Preferred Specimen | Plasma |
| Preferred Container | Citrate, 3.2% (Lt Blue) |
| Other Specimen/Container | Whole Blood in Citrate, 3.2% (Lt Blue) if testing is done within 24 hours. |
| Optimum Volume | Plasma: 1 mL Whole Blood: Entire Tube |
| Collection Instructions | Draw full tube Mix tube well. |
| Handling Instructions | Double centrifuge to produce platelet-poor plasma, avoid the WBC/platelet buffy layer, separate and freeze at -20 deg C immediately. Do NOT thaw once specimen is frozen. |
| Transport Requirements | Preferred method of transport is frozen plasma. |
| Specimen Stability | Ambient: 24 Hours Refrigerated: Not Stable Frozen: 2 Weeks Plasma |
| Rejection Criteria | Clotted, Contaminated, Hemolyzed, Improperly filled or underfilled tube, Collected in the wrong anticoagulant, Mislabeled, Specimen older than stability limits |
| Avail. Stat | NO |
| Analytic Time | Up to 1 Week |
| Methodology | Clot Detection |
Reference range(s)
The reference ranges listed below are valid on this date of August 31, 2025.
| Component | Age | Male Norm | Male Critical Low | Male Critical High | Female Norm | Female Critical High | Female Critical Low | Units | Add'l info |
|---|---|---|---|---|---|---|---|---|---|
| APCR | ALL | >2.24 | >2.24 | Results of... Note1 |
Note1:
Results of this assay may be altered in the presence of a lupus anticoagulant, direct thrombin inhibitor and Xa inhibitor drugs, and high levels of heparin (>1 U/mL). Genetic testing for the factor V Leiden mutation, rather than the APC-R screening assay, is recommended for patients with these conditions
.
